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These MM cells efficiently converted cADPR into ADPR and NAM, while the absent or low CD73 expression characteristic of malignant plasma cells accounted for the lack of detectable ADO formation

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The marrow was flushed with RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, or ImmunoCult-SF Macrophage Medium (Stem Cell Technologies) with 20 ng/mL murine M-CSF (PeproTech) to promote differentiation into macrophages