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The reactions were performed in a 25 l volume of KAPA SYBR FAST qPCR kit (Kapa biosystems, USA) with 10 picomole of forward and reverse primer from each gene ( Table 1 ) using Mx-3000P spectro flourometric thermocycler (Stratagene, USA)

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New confusion, weakness, poor oral intake, breathlessness, or reduced urine output in an older adult should be taken seriously regardless of temperature

doi: 10.1093/jnci/85.18.1492 160 QiaoYLDawseySMKamangarFFanJHAbnetCCSunXDet al

ISHIDA, T., Suzuki, T., Hirashima, S., Mizutani, K., Yoshida, A., Ando, I., Ikeda, S., Adachi, T., and Hashimoto, H