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Single-cell RNA sequencing Human kidney samples were prepared as single-cell suspensions according to the manufacturers instructions

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32 The positive association between frequency of MTHFR -C677T and MTHFR -A1298C polymorphisms and latitude documented in the current study suggests that in areas closer to the equator, which are increasingly exposed to UVR, activity of the MTHFR enzyme reflects greater functional competency because of the absence of the MTHFR polymorphisms

10.1038/s41598-020-78712-5 123 Salvador-BarberoB.lvarez-FernndezM.Zapatero-SolanaE.El BakkaliA.MenndezM