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Description

Dried discs were transferred to 96 deep-well blocks and treated, while shaking, with side-chain deprotection solution, consisting of 90% trifluoracetic acid (TFA), 2% dichloromethane (DCM), 5% H 2 O and 3% triisopropylsilane (TIPS) (150 L/well) for 1.5 h at room temperature (rt)

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This gene is expressed early during the HSV-1 replication cycle, prior to the entry of the viral genome into the host cell nucleus (54, 55)

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However, a similar movement was not observed in nucleosomes (data not shown)

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