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The regulatory mechanisms of TMAO-induced autophagy in different cells are shown in Figure 4

The release of damage associated molecular patterns (DAMPs) upon cell death, together with the release of reactive oxygen species (ROS) in oxidative bursts, further amplifies genotoxic cell stress ( Figure 2

Once the correct sequence of all the three genes was confirmed, they were subcloned into pET28a (Novagen), bacterial expression vector and pYES2 (Invitrogen), yeast expression vector, under the control of galactose inducible and glucose repressible GAL1 promoter

Treatment continued for 47 days until the largest tumour in the control group reached 2000 mm 3

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doi: 10.1038/s41467-019-09775-w 7 HuygheJRBienSAHarrisonTAKangHMChenSSchmitSLet al