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Studies demonstrate that when TLR5 / germ-free mice are infected with adherent-invasive Escherichia coli (AIEC) and subsequently exposed to a specific SPF microbial environment, they develop colitis symptoms (105)

Ca 2+ overload, which is a crucial event in eryptosis, leads to the opening of Ca 2+ -activated K + channel KCa3.1, known as the Gardos channel [90]

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Furthermore, the functional assessments of lower limbs of mice, including the reflexive mechanical pain-related responses experiment 34 and thermal pain-related responses experiment 35 , were conducted using the von Frey test and hot plate test, respectively