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Recall that Shc ultimately activates the MAPK pathway, while PLC has more metabolic effects including GLUT4 translocation

10.1093/cercor/bhz107 233 PuccaM

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After 30 min of incubation at room temperature in the dark, the solution was removed, and cells were washed with 3% BSA in PBS for 5 min and the nuclei were stained with Hoechst3332