When the culture had reached OD 600 0.4, 10 mL were each transferred to two 25 mL shake flasks, one of which was supplemented with 0.51 mM NH 2 -Pap
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Additional inclusion criteria at screening were a pre-bronchodilator RV of 130% of predicted normal, moderate-to-severe COPD according to GOLD guidelines [post-bronchodilator (following inhalation of ipratropium bromide 68 g) forced expiratory volume in 1 s (FEV 1 ) between 30% and 125% of predicted normal), two LAMAsaclidinium bromide 400 g or glycopyrronium 50 g QD (both delivered via DPIs) reduced hyperinflation and dyspnea
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