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It is particularly useful when the body cannot properly absorb vitamins through the digestive system, such as in malabsorption syndromes

1S2 "The use of human hepatocytes to select compounds based on their expected hepatic extraction ratios in humans

This type of application makes use of the ability of affinity chromatography to provide both high selectivity and strong binding for a given target, making it possible to often isolate this target in only one or a few steps even when it is present in a complex matrix [16]