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After incubation, cells were washed with PBS twice, re-suspended, and immediately submitted to flow cytometric analysis using a BD Accuri C6 Plus flow cytometer (Becton Dickinson, Mountain View, CA, USA)

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Urate-lowering therapy (allopurinol, febuxostat, uricosurics) reduces the amount of uric acid your body produces or increases how much it excretes, gradually dissolving existing urate crystals and preventing new ones from forming

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All patients were presented with ischemic stroke within 24 hours from symptom onset, and had relevant ischemic lesions on brain imaging