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In brief, the primers GDF15A 5-TCAACTTTAAGCCAGAAGGTGGCG-3, GDF15B 5-CTTCGGGGAGACCCTGACTCAGC-3 and GDF15D 5-ACTGCGAATCTAGAGAACCCTGAC-3 were used to amplify the targeted region

Xu J, Duan X, Hu F, Poorun D, Liu X, Wang X, et al

We also appreciate the Veterinary Department of Michael Okpara University of Agriculture, Umudike for providing the disease free experimental rats used in this study

Metabolic Benefits: Enhances lipid profiles and may improve insulin sensitivity, contributing to overall metabolic health

Next, cells were treated with the corresponding secondary antibodies, including Cy3 (1:250, Proteintech, SA00009-2) and FITC (1:250, Sigma, F0257) for 1 h in a dark room

Her concern is that appetite suppression can make it easier to under eat, especially when protein is not prioritized