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The impact of ROS on a cell is determined not only by their concentration within the cell but also through the equilibrium of ROS and endogenous antioxidants

Nrf2-mediated redox stress response signaling was compared between each group (HI, LI, & NC) as well as paired comparisons within animals unstimulated control and stimulated (exercised) limbs (Figure 1)

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10 mL of the cell culture were collected and filtered through the Whatman GF-C glass microfiber filters

Performance of the method The modified GPx-DTNB method's performance was completed following the bioanalytical method validation guidelines set by the Committee for Medicinal Products for Human Use (Singh and Narsinghani 2023)