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After washing, sections were incubated 30 min with horseradish peroxidase-labelled goat-anti-rabbit (GARPO) or rabbit-anti-goat (RAGPO) secondary antibodies diluted in 1% BSA/PBS + 1% normal human (AB) serum to stop unspecific binding

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Although in vivo treatment suggested that NMN could have an impact on tumour growth, in vitro experiments with a matrix of chemotherapy and cancer cell lines showed no impact of NMN on cell survivalsuggesting that any impact may be due to altered growth kinetics, rather than interfering with the efficacy of chemotherapy

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