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Finally, the coverslips were incubated for 1 hour in a humidified chamber at RT in secondary Abs (1:200, unconjugated from Jackson ImmunoResearch and conjugated in house as previously described (Coons et al., 1942), donkey anti-mouse IgG: cat# 715-005-151, RRID: AB_2340759

SNPs in three genes, namely, folylpoly--glutamate synthase ( FPGS ), -glutamyl hydrolase ( GGH ), and folate carrier ( SLC19A1 ), have been reported as potential predictors of toxicities and efficacy after therapy with pemetrexed [9, 10, 17]

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