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The cartilage particles were digested with collagenase type II (0.2% w/v in BM, Worthington Biochemical, Lakewood, NJ, United States) in a 37C shaker overnight

Science 294 : 13071313 Van Wyk JJ, Graves DC, Casella SJ, Jacobs S (1985) Evidence from monoclonal antibody studies that insulin stimulates deoxyribonucleic acid synthesis through the type I somatomedin receptor

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Lys105 and Tyr110 do not affect the thiol p K a value of Cys108 Based on studies on human Grx1 and NrdH from Corynebacterium glutamicum 40,41 , we previously suggested that Lys105 (residue K a in Fig