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Nat Rev Mol Cell Biol (2016) 17(11):691702

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After blocking for 1 h in 5% non-fat milk, membranes were incubated overnight at 4 C with anti-PC (1:1000, #16588-1-AP, Proteintech, Rosemont, IL), anti--actin, (1:5000, #sc-47778, Santa Cruz Biotechnology, Dallas, TX), anti-Nrf2 (1:500, #12721, Cell Signaling Technology, Danvers, MA), or anti-GPX4 (1:000, #14401933, Raybiotech, Peachtree, GA) antibodies

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