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Next, the team of researchers filmed what was happening just beneath the skull surface within five minutes of injury and so captured never-before-seen details of how the brain responds to TBI

PMID 25985235

This problem is rooted in the models structure

coli transformed strains: BL21(DE3)-RIL/pET30Bcp1, BL21(DE3)-RIL/pETC45S, BL21(DE3)-RIL/pETC50S were grown in selective medium and the expression of recombinant proteins were induced by IPTG