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Superior fluorescent probe for detection of cardiolipin

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Tatlidede E, Sehirli O, Veliolu-Onc A, Cetinel S, Yeen BC, Yarat A, et al

Enzymatic reactions were performed in 96-well plate format (Greiner Bio-One, 655101) with 190 l of reaction mix and 10 l of enzymes diluted in 10 mM sodium phosphate buffer pH 7.0 at the following concentrations: 1.36 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for ABTS, 1.7 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for DMP and guaiacol and 6.8 g ml 1 (LacAnc100) and 8.1 g ml 1 (LaccID) for sinapic acid

Maintaining adequate levels of this antioxidant is incredibly important

Biomed Pharmacother 2003;57(3-4):145-155