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Cells were transferred to conical tubes, pelleted by centrifugation at 1200 g for 5 min, resuspended in 1 ml of 1% BSA-DPBS, and counted using a brightfield hemacytometer

doi: 10.1074/jbc.M703713200 60 BersukerKHendricksJMLiZMagtanongLFordBTangPHet al

Interleukin-1 receptor antagonist delivered directly and by gene therapy inhibits matrix degradation in the intact degenerate human intervertebral disc: an in situ zymographic and gene therapy study