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Cells were fixed with 4% paraformaldehyde for 10 min, permeabilized using 0.1% Triton X-100 for 10 min, and blocked with 1% BSA solution for 1 h

These mixes provide strong proliferative and angiogenic effects but are often more expensive and less stable than peptide mixes

PCR analyses confirmed that the neuroprotective activity was mediated through modulation of the Wnt/ -catenin and PI3K/Akt/GSK-3 signaling pathways (91)

Cancer Control 31:111

Chromosomal regions with increased or decreased copy number in a test cell can be identified by detecting regions where the ratio of the two DNA signals is altered

This metabolic adaptation is critical because of the reduced entry of pyruvate into the TCA cycle by activated PDK1 and the increased lactate secretion in hypoxia 66