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After washing with TBST, membranes were incubated for 1 h at room temperature with the secondary antibodies conjugated to horseradish peroxidase (HRP) anti-rabbit IgG (Cell Signaling) or anti-mouse IgG (Cell Signaling)

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Acknowledgments EF is thankful to FAPESPFundao de Amparo Pesquisa do Estado de So Paulo (grant no