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Antigen retrieval was performed in sodium citrate buffer (10 mM, pH 6.0) at 80 C for 5 minutes, followed by washing in 1x PBS (pH 7.4) for 5 minutes at room temperature

The outcome measures were categorized from both the titles and the descriptions of the endpoint section of the records, based on previous investigations 61 and relevant core outcome sets

Step 4: Dissolve gently Remove the needle

In addition, the vaccine improved the physical activity of middle-aged mice (50 weeks old) and extended the median lifespan of ZMPSTE24 KO mouse (model of progeria), an extreme model of accelerated aging 62

This feature is particularly beneficial in fast-paced medical environments where time and resources are of the essence