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Pentose Phosphate Pathway: Oxidative Phase The overall reaction of the oxidative phase of the pentose phosphate pathway is shown below: Glucose 6-phosphate + 2 NADP+ + H2O Ribulose 5-phosphate + 2 NADPH + 2 H+ + CO2 In the first step of the oxidative phase, glucose 6-phosphate is oxidized to 6-phosphoglucono--lactone, which is a lactone or cyclic ester (Figure 1)

Steady state studies varying NADPH and inhibitor concentrations indicate that treatment with the fast inhibitors 7 and 8 or the slow inhibitor 3 , (the only slow inhibitor reaching equilibrium in 6 h), resulted in decreases in both K m and V max values consistent with uncompetitive inhibition of TGR, while treatment with 9 , a close analog of compound 8 synthesized ad hoc to facilitate structural studies (see below), changed only the V max indicating noncompetitive inhibition (Supplementary Tables 15 and 16)

Biological Psychiatry, 61 , 911922

Annu Rev Physiol (2017) 79:54166

doi: 10.3389/FIMMU.2017.01908 113 XuL.NirwaneA.YaoY

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