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Neurochemical and behavioral studies on ethanol and nicotine interactions

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However, note that although it is true that complete denaturation abolishes GFP fluorescence, recovery of fluorescence does not perfectly parallel the structure recovery, meaning that even a partial refolding could lead to fluorescence recovery, as demonstrated by urea denaturation at pH 6.2 slightly above the pI of GFP (pH 5.6), which does not appear to lead to full denaturation [27]

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