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Disclosures: Allison Carroll: Nothing to Disclose, Wolfram Goessling: Nothing to Disclose, Marc Sherman: Nothing to Disclose 2430 FXR AGONIST INT-787 INHIBITS INFLAMMATION, FIBROSIS, SENESCENCE, AND APOPTOSIS ARREST IN HUMAN CHOLANGIOCYTES MODELING PSC Francesca De Franco 1 Daniela Passeri 1 Sanjay Kansra 2 Luciano Adorini 2 Mary Erickson 2 Roberto Pellicciari 1 , 1 TES Pharma S.r.l., Perugia, Italy, 2 Intercept Pharmaceuticals, Inc., Morristown, New Jersey, USA Background: Cholangiocyte senescence is present in all stages of primary sclerosing cholangitis (PSC) and is associated with disease severity

Radiolabeled 32 PO 4 - is added to cells for one minute, which leads to the labeling of newly synthesized phospholipid (PL) located in the inner leaflet

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Abstract Background The insulin/IGF-1 signaling pathway has a major role in the regulation of longevity both in Caenorhabditis elegans and mammalian species, i.e., reduced activity of this pathway extends lifespan, whereas increased activity accelerates the aging process