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For Western blot analysis, the brain hemispheres were homogenized in a RIPA lysis buffer (10 mM TrisHCl pH 7.5 containing 150 mM NaCl, 1% Nonidet P40, 0.1% SDS, 1% Triton X-100, PMSF 0.1 mg/mL) supplemented with proteinase and a phosphatase inhibitor cocktail (1:100, Sigma Aldrich)

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AZD3956 has shown promise as a clinical candidate for reducing lactate levels in the TME, thereby facilitating immune cell infiltration

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CrossRef Morning blood pressure surge is associated with autonomic neuropathy and peripheral vascular disease in patients with diabetes Federica Di Gennaro, Cinzia DAmato, Roberto Morganti, Carla Greco, Susanna Longo, Diana Corradini, Davide Lauro, Vincenza Spallone Journal of Human Hypertension.2020