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Responses of marine animals to ocean acidification

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doi: 10.1016/j.anifeedsci.2021.114872 235 PoolsawatL.LiX.YangH.YangP.AmirulM.ChowdhuryK.et al

Total protein extracts (2040 g) were resolved by SDSPAGE under reducing conditions and transferred to polyvinylidene difluoride (PVDF) membranes, which were blocked with 5% non-fat milk or bovine serum albumin (BSA) in Tris-buffered saline with Tween-20 (TBST) to minimize nonspecific binding of primary antibodies, including rabbit polyclonal anti-FAM134B (1:1000