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In type 2 diabetes, insulin may be required on a long-term basis if other treatment options are ineffective or when insulin deficiency occurs

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This is how our team often visualizes the trade-offs when discussing protocol design with researchers

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The hemibrain pellets were immediately lysed in 100 L of 1% SDS buffer (1% SDS in IP Lysis Buffer supplemented with 1X protease inhibitor, 1X phosphatase inhibitor, 100mM N-Ethylmalamide), and homogenized with a pestle then boiled at 95 C for 10 minutes

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