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In accordance with the accumulation of lipid peroxides upon TCR stimulation, a significant decrease of GPX4, as assessed by Western blotting in various donors, was detected in subsequent weeks after stimulation (Fig

PLs were separated on a normal phase column (Luna 3 m Silica (2) 100 , 150 2.0 mm, (Phenomenex)) at a flow rate of 0.2 mL/min on a Dionex Ultimate 3000 HPLC system

This downregulation activated the NF-B signaling pathway (2.55 0.05-fold in HCT116 and 2.27 0.08-fold in DLD1), leading to upregulation of miR-155-5p (2.12 0.02-fold in HCT116 and 2.01 0.01-fold in DLD1) (60)

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