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Methods Cell culture RAW 264.7 cells (ATCC) were cultured in RPMI 1640 with 25 mM HEPES, 1% penicillin/streptomycin and 10% FBS (dialyzed FBS was used for the metabolite extraction experiments) at 37 C and 5% CO 2

Thus, changes in these biomarkers do not accurately assess real-time brain function since the underlying and established deficits in neuronal functioning are unaffected by their absence

However, efficacy may vary and multiple sessions are needed

In addition, it is clinically safe and non-toxic even at a high therapeutic dose [109]

Lancet 397 , 194 (2021)

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