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Materials and Methods Animal ethics All experiments were conducted strictly under the Guidance of the Care and Use of Laboratory Animals in China

This activation increases the transcription of MAFbx and MuRF1, enhancing the proteolytic activity of the UPS

Although the body can synthesise L-carnitine, we can also obtain it from certain foods

Remember that some lab-to-lab variability occurs due to differences in equipment, techniques, and chemicals used

2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions