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[5] Treat cells with this compound at desired concentrations (typically 10-100 nM) simultaneously with or prior to application of toxic insults (e.g., 10-40 M tert-butyl hydroperoxide or 1 mM 3-nitroproprionic acid)

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qPCR Primers: Protein synthesis assay Cells were cultured as indicated, washed twice with PBS, and changed to methionine/cystine/glutamine-free DMEM (Thermo, 21013024) supplemented with 0.5 mM L-cystine (Sigma, 57579) and L-glutamine (4 mM) with 50 Ci/mL 35 S-methionine (PerkinElmer, NEG009L005MC) for 20 min

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