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The conditions used were: 5 min at 95C and 40 cycles of: 1 min at 95C, 1 min at 55C and 30 s at 72C, followed by a melting curve (81 cycles of 10 s starting at 5C), which allows monitoring the dissociation kinetics of the amplified fragments

Melatonin represents a potential therapeutic approach for pSS owing to its capacity to regulate ferroptosis

Glutathione is often called the bodys master antioxidant and for good reason

After further washing with TBST buffer, the membrane was photographed using the chemiluminescent imaging system

10.1016/j.freeradbiomed.2004.04.034 [DOI] [PubMed] [Google Scholar] Ganai S