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Colony-forming units (CFUs) were recorded after overnight incubation at 37 C

[18] characterized microbial HAases (mHyals) as Mn 2 /Ni 2 -sensitive enzymes with optimal activity at 3745 C and pH 5.57.0 [54], mHyals serve as virulence factors that mediate bacterial invasion, tissue penetration, and dissemination [55, 56]