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A solution will have a lower freezing point than a pure solvent

Based on our experience, the plasma or serum sample has good stability when stored at -20 (more than 6 months) and pooled plasma samples as well as commercially available quality control sera can be used as in-house reference standard for cholinesterase activity after analyzing BChE by the proposed method

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Given evidence from both in vivo and in vitro studies suggests that the amino acid leucine may represent an effective trigger for muscle anabolism, consumption of foods rich in this amino acid may also be particularly important for maximising muscle protein synthesis (Reference Phillips and Van Loon32,Reference Breen and Churchward-Venne143)

doi: 10.1152/ajpcell.00225.2018 86