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Mice studies For all experiments, mice were acclimated for 1 week, followed by a 2-week co-housing period before the experiments started

Pharmacokinetics A set of 11 healthy male individuals (n=8 for NAD+ and n=3 for controls) participated in a pharmacokinetic study to examine how changes in NAD+ and its metabolite concentrations during NAD+ IV infusion affected the subjects' overall health