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Animals treated with NMN received a single injection at the time of chemotherapy, in addition to ongoing treatment with NMN in drinking water at 2 g/L, which started 72 h prior to chemotherapy and continued until the end of all experiments

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Assay: Glutathione in Sprague Dewley rats renal tissue

When ZFN binds to the target gene, FokI will cut the DNA to form a double-strand break, thereby triggering the cells repair mechanism and achieving gene editing (Fig

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