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Conventional biological nitrogen removal processes such as nitrification-denitrification are energy- and chemical-intensive, relying heavily on aeration and external organic carbon, while also producing high biomass and GHG emissions 170

In a pilot trial of NAD+ intravenous infusion in healthy adults, subjects receiving 750 mg of NAD+ over 6 hours reported no negative symptoms, and there were no vital sign abnormalities or serious laboratory derangements compared to placebo

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Hypotension and orthostatic hypotension were more frequently seen in patients on concomitant antihypertensive therapy

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