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Description

the membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

Where is the medication produced

Then, 50 L standard was added to the standard well, 50 L sample dilution buffer to the blank well, and 10 L sample with 40 L dilution buffer to the sample well