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& Gianfrancesco, G.) (Springer, 2009)

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Lentivirus (LVS), another CRISPR-Cas9 vector, has larger cloning efficiency (8KB) than the AVV vector, allowing sgRNA and Cas9 to be cloned into a single LV vector

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In addition, secreted roGFP2-Orp1 (or other pH-stable fluorescent proteins) can provide valuable information regarding zones of secreted protein retention and diffusion barriers in different plant species and tissues