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A novel small molecule Hsp90 inhibitor, C-316-1, attenuates acute kidney injury by suppressing RIPK1-mediated inflammation and necroptosis

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Total GSH is measured at an absorbance of 412 nm as the oxidized GSSG is reduced to two GSHs by NADPH, Glutathione reductase, and the reduced GSH and GSH present in the existing sample react with DTNB to form yellow TNB

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For instance, Fisher et al.[48] found up to 7-fold differences in the rates of APAP glucoronidation in a sample of 20 human livers, and Court et al.[54] found 15-fold inter-individual variability in APAP glucoronidation rates in liver microsomal fractions