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doi:10.5664/jcsm.5870

The melanoma-upregulated long noncoding RNA SPRY4-IT1 modulates apoptosis and invasion

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2.6 Real-time PCR analysis Total RNA was prepared from liver tissues and LO2 cells using the simple RNA Extract kit (Tiangen Biotech, China) and reverse transcription of RNA to cDNA using Fast King cDNA First Strand Synthesis Kit (Tiangen Biotech, China)