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However, the binding affinities of EGCG to HIF-1 and many other targets are higher than the EGCG plasma peak level in experimental animals administered with high dose of EGCG, raising a concern whether the microRNA regulation by HIF-1 is involved in the anti-cancer activity of EGCG in vivo

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In most cases of POAG, the disease may progress to total blindness without the patients experiencing any pain or discomfort, which however may occur when the rate of tension is very high, and IOP exceeds ~50 mm Hg

The fixative was removed and 150 l of Nile Red solution (6 l of Nile Red 0.5 mg/ml in acetone per 1 ml of 40% isopropanol) was added to the worms for 30 min at 20 C with gentle rocking in the dark

with Hsp70 minimal promoter, Mhc intron and SV40 polyA sequences used as described for the refinement of tools for targeted gene expression in Drosophila [26]