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To probe the involvement of ERO1 in ferroptosis, we studied the effect of EN460, a known ERO1 inhibitor [42], on erastin-induced ferroptosis

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Preferred sites: Abdomen (avoid 2 inches around navel) Front or outer thigh Upper arm (posterior) Technique: Clean injection site with alcohol swab Let alcohol dry completely Pinch fold of skin Insert needle at 45-90 degree angle Inject slowly over 5-10 seconds Release skin, withdraw needle Light pressure if any bleeding Rotate sites to prevent tissue irritation