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NTS prepared as previously described and cultured in M199 supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum were incubated at 37 C in a clear bottom flat well plate in a 5% CO 2 incubator overnight

In fact, selenite, the most common form of dietary inorganic selenium, is rapidly transformed to selenide and enters this metabolic pathway directly through that selenide door. One can understand that it is then rapidly incorporated in the seleno-cysteinyl moiety and activates Se-GSH-Px

controls ( p = 0.033) is not observed in the de novo patients vs

Therefore, this combinatorial approach for cysteine protease inhibitor screening can be extended to other disease-relevant enzymes 59

Cailotto C, Costes LMM, van der Vliet J, VAN Bree SHW, VAN Heerikhuize JJ, Buijs RM, et al