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Cysteine is a pivotal organic compound that contributes for cancer metabolic remodeling at three different levels: (1) in redox control, free or as a component of glutathione

Plant Physiol 61:119121

J.AlamiA.AlexanderG

Briefly, the authors developed a stable clone by transfecting MCF7 cells with the cDNA of human cytosolic GPx1

After incubation an aliquot of each sample was assayed for M pro activity (1 M) and analyzed (2 L) by SEC/MALDI-TOF to determine the percent of glutathionylation in each treatment based on the abundances of each species