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The priming step is induced by TLRs and cytokine receptors, such as TNF receptor or IL-1 receptor, and then NF-B is activated and upregulates transcriptionally NLRP3 and pro-IL-1, which promotes NLRP3 to form inflammasome assembly

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For the 4-week duration of the acclimation phase, cats were offered a control dry diet (IAMS Adult Cat Original Chicken, Mars Petcare)