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Membranes were blocked with 5% milk in Tris-buffered saline with Tween-20 (TBST) and then incubated with primary antibodies overnight at 4 C (all antibodies were 1:1,000 in 5% milk in TBST, while GAPDH primary antibody was diluted 1:5,000 in 5% milk in TBST)

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This disorder has various causes, such as genetics or secondary to dialysis treatments for chronic kidney disease

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This study demonstrates that the iron in myoglobin can be replaced with various noble metals, resulting in activity for new reactions