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Western blots Cell lysates and supernatants (reduced and denatured) were separated on 412% gradient gels (Invitrogen), proteins transferred onto nitrocellulose membrane (Amersham), and ponceau staining performed routinely to evaluate loading accuracy

However, HCC recurrence is the main reason leading to the poor overall survival of HCC recipients after LT due to the lack of effective prediction and prevention strategies for HCC recurrence [2]

These triggers may include temperature changes, certain cosmetic ingredients, ultraviolet light, wind, pollution, or even emotional stress

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Insufficient oxygen, oxidative stress, and swelling are the causes of this inflammation [39, 43]