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JAMA 328 , 18491861 (2022)

Peptide lysates were injected on a trapping column (Acclaim PepMap 100 C18, 3 m, nanoViper, 75 m x 2 cm, Thermo Fisher Scientific) with 5 L/min by using 98% water/2% ACN 0.5% trifluoroacetic acid, and separated on an analytical column (Acclaim PepMap 100 C18, 3 m, nanoViper, 75 m x 25 cm, Thermo Fisher Scientific) with a flow rate of 300 nL/min

These ideas rely on data drawn from other studies

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The research discussed involves primarily animal studies, and human clinical data remains limited